Prof. Rishpon Judith

  
Affiliation:Biotechnology, The George S.Wise faculty of life sciences
Tel:  (972)-3-6409366
(972)-3-6407525
 
Fax: (972)-3-6409407
 
Email: judithri@tauex.tau.ac.il
 
Personal Website:

 
Postal Address:Biotechnology
The George S.Wise faculty of life sciences

Tel Aviv University
Tel Aviv 69978

Research Interest

Application of nano technologies in electrochemical Biosensors
Carbon nanotubes on amperometric electrodes
We have effectively exploited the unique electronic properties of carbon nanotubes (CNT) in electrochemistry as a means of promoting the electron transfer reaction for the development of enzyme based sensors. CNT were attached to gold or carbon electrodes and applied in a sensitive detection of hydrogen peroxide employing the enzyme horse reddish peroxidase immobilized on a CNT modified electrode. This sensor was capable to measure enzymatic activity released from the mycobacteria smegmatis (a model system for mycobacteria tuberculosis). CNT attached to electrodes were also exploited in a highly sensitive electrochemical enzyme immunosensors.

Peptide nanotubes on amperometric electrodes
In addition, we have examined the possibility of employing peptide nanotubes (PNT) as catalytic elements in amperometric biosensors [1]. Voltammetric and time based amperometric techniques were applied to demonstrate the significantly improvement electrochemical parameters by the PNT. These findings clearly show that this novel class of peptide nanotubes provides an attractive component for future electroanalytical biosensors.

Lipid nanolayer on gold electrode
We have investigated interactions between receptors and hormone by following the impedance changes in 5-7nm thick lipid bilayers on gold electrodes. The system respond to estrogen or testosterone at physiological concentrations. Moreover, it enables the detection of xenohormones like xenoestrogens that are a health risk in the environment [2].

Lab on a Chip
We developed an innovative electrochemical ‘lab on a chip’ system that integrates the applicability of physiological reactions to serve as biosensors with the advantages of micro electro mechanical systems (MEMS). The novel specific design and process of the nano-biochip adjusted to an exclusive biochemical process enables highly accurate, sensitive and rapid diagnosis of physiological reactions by a hand held miniaturize device [3]. This system was used in the detection the response of microorganisms to acute toxicity in water.

 

Selected Publications


  • M. Yemini, P. Xu, J. Rishpon, and D. Kaplan, Collagen-like Peptide as a Matrix for Enzyme Immobilization in Electrochemical Biosensors. Electroanalysis. Vol 18, (21) 2047-2054, 2006.
  • R. Popovtzer, T. Neufeld, E. z. Ron, J. Rishpon, and Y. Shacham-Diamand, Electrochemical detection of biological reactions using a novel nano-bio-chip array, Sensors and Actuators B: Chemical, vol. 119 pp.664-672, 2006.
  • T. Neufeld, D. Biran, R. Popovtzer, T. Erez, E. Z. Ron, and J. Rishpon, Genetically engineered pfabA pfabR bacteria: an electrochemical whole cell biosensor for detection of water toxicity, Analytical Chemistry, vol. 78, pp. 4952-4956, 2006.
  • M. Yemini, M. Reches, E. Gazit, and J. Rishpon, Peptide nanotube-modified electrodes for enzyme-biosensor applications, Analytical Chemistry, vol. 77, pp. 5155-5159, 2005.
  • V. Lirtsman, R. Ziblat, M. Golosovsky, D. Davidov, R. Pogreb, V. Sacks-Granek, and J. Rishpon, Surface-plasmon resonance with infrared excitation: Studies of phospholipid membrane growth, Journal of Applied Physics, vol. 98, 2005.
  • M. Yemini, M. Reches, J. Rishpon and E. Gazit, Nano letters 5: 83-186 (2005).
  • R. Popovtzer, T. Neufeld, D. Biran, EZ. Ron, J. Rishpon, and Y. Shacham-Diamand Nano Letters 5, 1023 - 1027 (2005).
  • V Sacks-Granek and J. Rishpon, Environ. Sci Tech., 36, 1574-1578 (2002).