Prof. Korenstein Rafi

  
Affiliation:Physiology - Pharmacology, Sackler School of Medicine
Sackler building
Tel:  (972)-3-6406042
(972)-3-6406042
 
Fax: (972)-3-6408982
 
Email: korens@tau.ac.il
 
Personal Website:

 
Postal Address:Physiology - Pharmacology
Sackler School of Medicine

Tel Aviv University
Tel Aviv 69978

Research Interest

Electrical enhancement of drug nano-carriers; Nanoscale cell membrane dynamics

The activity of research group in the field of nanoscience and nanotechnology addresses two main topics: (1) Electrical enhancement of delivery of nanoparticles carrying drugs into cells (electroendocytosis) and toxicology of nanoparticles; (2) Nanoscale dynamics of the cell surface of cells. In order to achieve more effective therapies while eliminating the potential for both under- and overdosing, we have developed a novel methodological platform to induce highly efficient transfer of specifically designed nanoparticles carrying drugs into cells. The method is based on exposure of cells to trains of low unipolar electric fields which leads to an efficient uptake of macromolecules and nanoparticles into cells. One application of this methodology is the treatment and cure of solid tumors in mice bearing different types of metastatic cancer.
The study of nano-scale local dynamics of the cell surface addresses two major directions (i) Understanding the relationship between the molecular structure of the membrane-skeleton complex and nano-scale cell membrane fluctuations under physiological and pathological situations; (ii) The non-linear analysis of time series of cell membrane fluctuations and their use as specific "signature" patterns for diagnosis employing a "cell on chip" configuration. This study involved the development of novel methodologies to monitor nano-scale cell membrane fluctuations.

Selected Publications


  • Plotnikov A, Tichler T, Korenstein R, and Keisari Y. (2005) Involvement of the immune response in the cure of metastatic murine CT-26 colon carcinoma by low electric field enhanced chemotherapy. Int. J. Cancer 117: 816-824 (2005)
  • Antov, Y. Barbul, A.Mantsur H.and Korenstein, R (2005) Electroendocytosis: Exposure of cells to pulsed low electric fields enhances adsorption and uptake of macromolecules. Biophys J. 88: 2206-23.
  • Goldstein M, Leibovitch I, Levin S, Alster Y, Loewenstein A, Malkin G, Korenstein R. (2004) Red blood cell membrane mechanical fluctuations in non-proliferative and proliferate diabetic retinopathy. Graefes Arch Clin Exp Ophthalmol. 242: 937-43.
  • Scheffer, L., Bitler, A., Ben-Jacob, E. and Korenstein, R. (2001) Atomic Force Pulling: Probing the local elasticity of the cell membrane. Eur. J. Biophys. 30:83-90.