Prof. Freeman Amihay

Affiliation:Biotechnology, The George S.Wise faculty of life sciences
Tel:  (972)-3-6409054
Fax: (972)-3-6405147
Personal Website:

Postal Address:Biotechnology
The George S.Wise faculty of life sciences

Tel Aviv University
Tel Aviv 69978

Research Interest

Biologically active metal-enzyme and metal-cell hybrids and their integration with electrodes and biochips
New methods were developed for the fabrication of novel enzymatically active, soluble and stabilized enzymes and binding proteins for their integration with electrodes and biochips.
This project is carried out in collaboration with Prof. Shacham from The Department of Physical Electronics, Faculty of Engineering, and Prof. Rishpon from our department.      

Protein crystals as biotemplates for the fabrication of novel composite materials
The use of protein crystals as highly ordered biotemplate enabling "filling" of its nano-structured voids array was established. Novel approaches for restructuring of the porosity of a given protein crystal were developed along with methods for the stabilization of the protein biotemplate and monitoring of the filling process were demonstrated.

This project is carried out in collaboration with Prof Frolow from our department.

Silver-glucose oxidase hybrid

Crystal porosity modification by site-directed mutagenesis

Selected Publications

  • Wine Y, Cohen-Hadar N, Lamed R, Freeman A, Frolow F (2009). Modification of protein crystal packing by systematic mutations of surface residues. Biotechnology and Bioengineering 104: 444-457.
  • Vernick S, Moscovich-Dagan H, Porat-Ophir C, Rishpon Y, Freeman A, Shacham-Diamand Y. (2009). Directed metallization of single enzyme molecules with preserved enzymatic activity. IEEE Transactions 8: 95-99.
  • Cohen-Hadar N, Wine Y, Lagziel-simis S, Dror Y, Frolow F, Freeman A (2009). Protein crystal mediated biotemplating. J Porous Media 12: 213-220.
  • Ben Yoav H, Freeman A. (2008). Enzymatically attenuated in situ release of silver ions to combat bacterial biofilms. J Drug Delivery Science and Technology 18: 25-29.
  • Wine Y, Cohen-Hadar N, Freeman A, Frolow F (2007). Elucidation of the mechanism and end products of glutaraldehyde crosslinking reaction of protein crystal by X- ray structure analysis. Biotechnology and Bioengineering 98: 711-718.
  • Moscovich-Dagan H, Cohen - Hadar N, Porat C, Rishpon J, Shacham-Diamand Y, Freeman A. (2007). Wiring of the catalytic site of novel molecular enzyme-metal hybrids to electrodes. J Physical Chem C 111: 5766-5769.
  • Lagziel-Simis S, Cohen - Hadar N, Moscovich-Dagan H, Wine Y, Freeman A (2006). Protein mediated nano-scale biotemplating. Currents Opinins in Biotechnology, 17: 569-573.
  • Cohen-Hadar N, Wine Y, Nachliel E, Huppert D, Gutman M, Frolow F & Freeman A (2005). Biotemplating of synthetic gels by stabilized protein crystals. Biotechnology and Bioengineering, 94: 1005-1011.
  • Schacham-Diamand Y, Inberg A, Sverdlov Y, Croitoru N, Moskovich H & Freeman A (2003). Electroless processes for micro and nano-electronics. Electrochim Acta 48: 2987-96.
  • Dotan N, Cohen N, Kalid O & Freeman A (2001). Supramolecular assemblies made of biological macromolecules. In Nanosurface chemistry (Rosoff N.,Ed) pp 461-471, Marcel Dekker.
  • Dotan N, Arad D, Frolow F & Freeman A (1999). Self-assembly of a tetrahedral lectin into predesigned diamond-like protein crystals. Angewandte Chemie Int Ed 38: 2363-6.