Prof. Shaked Natan T.

Affiliation:Department Of Biomedical Engineering, Faculty of Engineering
Tel:  (972)-3-6407100
Fax: (972)-3-6407100
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Postal Address:Department of Biomedical Engineering
Faculty of Engineering

Tel Aviv University
Tel Aviv 69978

Research Interest

  • Biomedical Optical Microscopy and Nanoscopy.
  • Optical Imaging and Therapy using Nano-Particles in Biological Cells.
  • Optical Interferometric Systems (including Interferometric Phase Microscopy, Optical Coherence Tomography and Endoscopic Interferometric Systems).
  • Optical Signatures of Cancer and Alzheimer's Disease.
  • Three-Dimensional Optical Imaging and Holography for Biomedical Applications.

Microscopic biological specimens, such as biological cells, are dynamic objects, continuously adjusting their three-dimensional sizes, shapes and other biophysical features. Microscopy and nanoscopy of these specimens can provide a powerful research tool for cell biology studies, as well as a means for medical diagnosis and monitoring of diseases. Many biological specimens, however, are mostly-transparent objects, and thus imaging them with conventional bright-field light microscopy fails to provide adequate image contrast. For this reason, exogenous contrast agents such as fluorescent labels are widely used in biomedical microscopy. However, these exogenous agents might be cytotoxic in the long run and there is a possibility that they will influence the specimen behavior. Additionally, fluorescent agents tend to photobleach, potentially limiting the imaging duration.
Novel optical interferometric and spectroscopic methods are able to uniquely characterize biological cells in vitro or in vivo, without using any contrast agents or sample preparation. For example, interferoemtric phase microscopy using low coherence light sources provides a fully quantitative tool for recording the three-dimensional structure of cells, as well as the extremely fast dynamics associate with them (with rates of up to several thousands of full frames per second), where the accuracy of measurement in the axial dimension can be sub-nanometric. These low-cost and high-accuracy methods have been shown to be sensitive enough for early detection of diseases such as cancer and Alzheimer's disease. Plasmonic metal nano-particles that are adequately excited by external sources are able to add specificity to the interferometric measurements, which enables nano-tracking, biological imaging below the diffraction limit, and directed nano-therapy. These novel techniques have a great potential as diagnostic tools for clinical applications, and thus we also examine the integration of the proposed techniques into a novel endoscopic fiber bundle probes, enabling in-vivo measurements and clinical implementations.

Selected Publications

  • N. T. Shaked, L. L. Satterwhite, G. A. Truskey, M. J. Telen,and A. Wax, "Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry," Journal of Biomedical Optics Letters, Vol. 16, No. 3, 030506, 2011.
  • Y. Zhu, N. T. Shaked, L. L. Satterwhite, and A. Wax, "Spectral-domain differential interference microscopy," Optics Letters, Vol. 36, Issue 4, pp. 430-432, 2011.
  • N. T. Shaked, L. L. Satterwhite, N. Bursac, and A. Wax, "Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy," Biomedical Optics Express, Vol. 1, Issue 2, pp. 706-719, 2010.
  • N. T. Shaked, J. D. Finan, F. Guilak, and A. Wax, "Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry," Journal of Biomedical Optics Letters, Vol. 15, Issue 1, 010505, 2010.
  • N. T. Shaked, M. T. Rinehart, and A. Wax, "Dual interference channel quantitative phase microscopy of live cell dynamics," Optics Letters, Vol. 34, Issue 6, pp. 767-769, 2009.